Doncaster Mile favourite Kementari will start from barrier two in the 16-horse field for the $3-million race.
The Godolphin colt will be ridden by Golden Slipper-winning jockey Brenton Avdulla on Saturday.
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Happy Clapper, runner-up in the past two Doncasters, will start from barrier one.
Mister Sea Wolf, one of five runners for trainer Chris Waller, has drawn the outside gate.
Arbeitsam will start from barrier 10 in his bid to give Gai Waterhouse a record eighth win in the Doncaster.